Objective Borrelia burgdorferi were isolated from captured ticks in the border areas between Guangxi and Guizhou, in order to identify the vector of Lyme disease in Guangxi Zhuang Autonomous Region and Guizhou province. Methods The BSK?Ⅱ culture media were used to isolate B. burgdorferi from ticks. The 5S-23S rRNA gene spacer region of the isolates was amplified by the PCR, and the product was cloned and sequenced. The sequencing results were compared with the 5S-23S rRNA gene spacer region of the B. burgdorferi strains from the gene bank and analyzed for the homology to identify their genotypes. Results One B. burgdorferi was isolated from the captured Ixodes sinensis (named QLT1). The isolate was identified as the genotype B. valaisiana. Conclusion I. sinensis is the likely vector of Lyme disease in Guangxi Zhuang Autonomous Region and Guizhou province.

【Abstract】 Objective To study the influence of season, temperature and humidity to the seasonal fluctuation of Haemaphysalis bispinosa.  Methods Investigate 3 d in succession in the fixed sites per ten days monthly, the ticks were captured by the flag (90 cm×60 cm) at its active peak. The average quantity of ticks captured in successive 3 d per ten days was counted, and the temperature and humidity were recorded. The curve of average ticks number to time was drawn. Results The ticks captured belonged to H.bispinosa. Its density began to increase since April, and it had two population peak, one was in May and June, the other was in September and October.  After October, it decreased obviously, and its density was minimum in February and March. The activity peak of ticks was perhaps from May to June, and its propagation peak was from September to October. The curve of temperature change and the seasonal fluctuation of H.bispinosa was very similar, so the combination of humidity and temperature possibly affected the activity of H.bispinosa. Conclusion H.bispinosa could be active in the whole year, and the Summer and the Autumn were its activity peak.

Objective To analyse prevalence trend of Japanese encephalitis(JE) in 1997-2004 in Guizhou province and to supply scientific gist for the prevention and Control strategy. Methods Analysis information was got from the epidemic surveillance and JE Cases Questionnaire. Results There were accumulative total 13 664 JE cases and 540 death cases. The annual average Morbidity and Mortality and fatality rate were 4.65/100 000 and 0.18/100 000 and 3.95%,respectively. Male to female was 1.48∶1. Children of decentralization resident population accounted for 67.54%( 9 228/ 13 664) and students were 23.06%( 3 151/ 13 664). Younger than 10 years old children case accounted for 88.00%( 12 024/ 13 664);Season distribution was from June to October and high peak period was from July to August. Conclusion The results showed that JE cases were mainly younger than 10 years old children in Guizhou province. The cases season were from June to October and high peak period lasted two months from July to August. The high risk prefectures were Zunyi and Bijie. There were so many cases with out vaccine history and unknown history during 1997-2004. The preventive strategies are to be that the health education and propaganda should be conducted. All-around control countermeasure such as preventive and destroying mosquitoes and JE vaccine immunization should be carried on to decrease the morbidity and mortality of JE in Guizhou province.

Objective Analyses epidemiological status of rabies and searches for the control countermeasure according to the rabies cases surveillance in Guizhou province in 2004.Methods Analyse synthetically information of epidemic situation surveillance administration system and rabies cases questionnaire form.Results Totally 206 rabies cases were reported in Guizhou province in 2004 and the morbidity was(0.53/100 000).Mortality was the same as morbidity.Among them,the rabies cases of Anlong county is account for the 65.67%((44/67)) of Qianxinan prefecture.Though there were rabies cases in the whole year,the peak was March and from July to October and the rabies cases from July to October amount for 46.12%((95/206).Proportion of male and female was 2.68∶1.The most cases were farmers and students which amount to 57.28% and 27.18% respectively.The high risk age was(10-44) years and amount to 66.5%.The youngest rabies case was 2 years old and the oldest was 76 years old.Conclusion Rabies cases mostly were farmers and students in Guizhou.The high peak season of rabies cases is summer and autumn.High risk prefecture is Qianxinan,Qiannan,Qiandongnan and Anshun city.Suggestion more works should be done according to "Administrating,Immunizing and Destroying" principle.Strengthen propaganda and health education,and let people realize the importance of injecting the rabies vaccine and immunize on one's own initiative.It will decline the rabies morbidity and mortality in Guizhou province and protect the health of people.

Objective:To describe the relationship between different types of epidemic field.Methods: Hantavirus infection was studied with epidemic surveillance methods in Apodemus agrarius population within Rattus type epidemic areas.Results:It showed that no Hantavirus were found in Apodemus agrarius of Rattus type epidemic areas.Conclusion:This indicated that the Rattus type HFRS epidemic areas was not revolted by Apodemos type.

The surveillance was based on the large scale of inverstigation and reseach in Guizhou,by means of combining the steady spots(1984-94; five counties) with the movable spots(1990-95;twenty nine counties). The results showed that: Apodemus agrarius and Rattus norvegicus Berkenhout were extensively prevalent in Guizhou.The former occupied a dominant position in agrarian fields in sixty two counties norih to twenty six degree north latitude and divided into two subspecies:A.a.Mantchuricus in the majority areas as Zunyi etc. and A.a.Ningpoensis in the minority areas of Guizhou as Liping etc. Rattus norvegicus was predominant in residencial areas in Guizhou.Both were main host animals and infectious sources: Apodemus agrarius in the middle, north and east-north part, while Rattus norvegicus in the east-south part of the province. Their density and virus carring rates varied in certain seasons and certain years. HFRS could be effectively controlled by wiping out the host animals and infectious sources.

Seras of 402 healthy persons from five faunae of Guizhou province were tested for antibodies against arbovirus (3 virus antigens of group A, 8 virus antigens of group B) by the method of hemagglntination inhibition(HI) test. The results in brief are listed below:1.Group A and group B arbovirus antibodies were found; 2.Japanese encephalitis antibody was widely distributed; 3.Dengue fever antibody only found in east-southern and southern parts of Guizhou;4.Group A arbovirus antibody positive rate was 31.68%(102/322),they were antibodies against MAY or related virus; group B antibody positive rate was 64.74%(202/312),they were: JE 24.40%(91/373), MVE 27.36%(110/402),DEN-38.01%(25/312).KUN 15.31%(49/320),KFD 35.15%(71/202), LGT 2.48%(5/202), and POW 28.36%(114/402). The levels of against arbovirus antibodies were 1:20 to 1:160,there were 7.76% ≥1:80 in group A and 69.90% in group B.It's known that cases of Japanese encephalitis were wide-spread as well as it's antibody found in this article but the other arboviruses infection maybe existed as discovery above mentioned.